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gary yellen  (Addgene inc)


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    Structured Review

    Addgene inc gary yellen
    Gary Yellen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+peredox+mcherry/pcDNA3%2E1-Peredox-mCherry+(Plasmid+%2332383)/10__1113_slash_jp288960-89-8-10
    Average 93 stars, based on 24 article reviews
    gary yellen - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Metabolic modulation of CtBP dimeric status impacts the repression of DNA damage repair genes and the platinum sensitivity of ovarian cancer
    Article Snippet: .. HEK293 cells were transfected with pcDNA3.1-Peredox-mCherry (addgene 32383 or 32384) plasmid, then the cells were treated by indicated drugs. .. Peredox and mCherry fluorescence signal were captured with Zeiss LSM710 confocal microscopy.

    Article Title: Nicotine rebalances NAD + homeostasis and improves aging-related symptoms in male mice by enhancing NAMPT activity
    Article Snippet: The total antioxidant capacity and CuZn/Mn-SOD activity was determined according to instructions provided with the Total Antioxidant Capacity Assay Kit and Cu/Zn-SOD and Mn-SOD Assay Kit purchased from Beyotime. .. HT22 cells were transfected with NAD + -NADH redox states fluorescent biosensor : pcDNA3.1-Peredox-mCherry (Addgene: #32383) and pcDNA3.1-Peredox-mCherry-NLS (Addgene: #32384). .. The plasmids were transfected with Lipofectamine 3000 according to the manufacturer’s instructions (Invitrogen) to HT22 cells in triplicates.

    Plasmid Preparation:

    Article Title: Metabolic modulation of CtBP dimeric status impacts the repression of DNA damage repair genes and the platinum sensitivity of ovarian cancer
    Article Snippet: .. HEK293 cells were transfected with pcDNA3.1-Peredox-mCherry (addgene 32383 or 32384) plasmid, then the cells were treated by indicated drugs. .. Peredox and mCherry fluorescence signal were captured with Zeiss LSM710 confocal microscopy.

    Article Title: Human peroxisomal NAD + /NADH homeostasis is regulated by two independent NAD(H) shuttle systems.
    Article Snippet: .. pcDNA3.1-Peredox-mCherry was obtained via Addgene from Gary Yellen (Addgene plasmid # 32383). .. To generate Peredox-PTS1, the stop codon of Peredox was replaced by the carboxy terminus of ACOX3, including its peroxisomal targeting signal (PTS1), preceded by a linker GSGGE (GSGGENKPVIGSLKSKL*) using a site-directed mutagenesis kit (NEB, E0554S) and primers (5’-acaaacctgtcataggaagtctgaaatcgaagctctagagtcgagaagcttaagtttaaaccgc-3’, 5’-acttcctatgacaggtttgttttcacctccggaaccagccttgtacagttcgtccatgccgcc-3’).



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